Western blot analysis of BNIP2 in extracts of various cell lines. Samples were incubated with BNIP2 Polyclonal antibody (Product # PA5-92864) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunocytochemistry-Immunofluorescence analysis of BNIP2 was performed in HeLa cells using BNIP2 Polyclonal Antibody (Product # PA5-92864).
Supplier Page from Thermo Fisher Scientific for BNIP2 Antibody